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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Development of the New Methods for Ecological Study of Soybean Bacterial Pustule : An Artificial Inoculation Method of Soybean Seed with Xanthomonas campestris pv. glycines ผู้เขียน: ดร.สุดฤดี ประเทืองวงศ์, ศาสตราจารย์ ,  Khanitha Khandej สื่อสิ่งพิมพ์:pdf AbstractAn attempt was made to develop the artificial inoculation method of seed transmission experiments. Injection of Xanthomonas campestris pv. glycines through seed mycopyle at 103 and 105 cfu/seed gave 100% seed transmission with no effect on seed germination. When seeds derived from artificially inoculated soybean plants were used, the rate of seed transmission was dependent on the disease severity of parent plants. Seed transmission ratio was higher with seeds taken from plants inoculated 3 times than those inoculated 2 and 1 times respectively. The administration of the vacuum pump (20 lb/inch2) for 10, 20 and 30 min after soaking seeds in bacterial suspenssion at 106 cfu/ml revealed no difference in percent seed transmission. Furthermore, this method significantly reduced seed germination. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : A Detached Leaf Culture Technique for Resistance Screening Against Bacterial Pustule in Soybean ) ผู้เขียน:Preecha Surin, Nalinee Srivakorn, Bruce Imrie, Robert Lawn สื่อสิ่งพิมพ์:pdf AbstractA series of experiments was conducted to obtain information on variation in virulence of Xanthomonas campestris pv. glycines, the causal organism of bacterial pustule, resistance of the soybean (Glycine max) host, and methods of screening for resistance. Variation was found in both virulence and resistance. It was concluded that, given the levels of variability detected, use of a detached leaf technique with inoculation by a pinprick method would be the most effective means of selecting for resistance. |
 ที่มา:การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 47หัวเรื่อง:กลไกใหม่ของเชื้อแบคทีเรียปฏิปักษ์ในการยับยั้งเชื้อสาเหตุโรคใบจุดนูนถั่วเหลือง |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Extracellular Proteome of Bacillus amyloliquefaciens KPS46 and Its Effect on Enhanced Growth Promotion and Induced Resistance Against Bacterial Pustule on Soybean Plant) ผู้เขียน:ณัฐธิญา เบือนสันเทียะ, ดุสิต อธินุวัฒน์, Tiyakhon Chatnaparat, Gary Y. Yuen, ดร.สุดฤดี ประเทืองวงศ์, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractBacillus amyloliquefaciens strain KPS46 is gram positive that has been found to be effective in controlling various diseases and improving crop yield under greenhouse and field conditions. This study was investigated for KPS46 ability to activate extracellular protein elicitors in enhanced plant growth and induced systemic resistance of soybean plants. Soybean cv. Spencer seeds treated with KPS46 increased root and shoot length, biomass by more than 20-40% and the reduced bacterial pustule disease severity up to 50% when compared with the non-treated control. Bioassay data showed that seedling growth phenotype increased by KPS46 was significant upregulation of 20- elicited extracellular protein spots, whereas sterile distilled water had no effect on either enhanced plant growth or induced resistance. To investigate the proteins involved in growth promotion and induced resistance mechanisms, two-dimensional gel electrophoresis was used to separate extracellular proteins secreted by KPS46 wild type and by N19G1, a UV-derived mutant of KPS46 with reduced production of extracellular proteins and lacking growth promotion and induced resistance activity. Using the 2D-PAGE, the extracellular protein of B. amyloliquefaciens KPS46 grown in nutrient glucose broth was studied. The identity and putative function of twenty proteins secreted by KPS46 but not by N19G1 were determined. The 20 extracellular protein spots were identified that were known to be secreted by various mechanisms. These extracellular proteomes of the strain KPS46 included proteins from different functional classes and some proteins of yet unknown function. To our knowledge, this was the first two-dimensional extracellular proteome map of a PGPRs, KPS46. The analysis revealed a number of proteins which might be involved in plant growth promotion and induced resistance by acting as plant growth regulators, accumulating bio-fertilizer/ nutrients, producing antibiotic compounds, stimulating metabolism or functioning in defense against stress factors. |
ที่มา:การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 47หัวเรื่อง:กลไกใหม่ของเชื้อแบคทีเรียปฏิปักษ์ในการยับยั้งเชื้อสาเหตุโรคใบจุดนูนถั่วเหลือง |